| 质粒名称: | pGL3-mArg1 promoter/enhancer -31/-3810 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 34571 |
| 存储实验室: | Peter Murray |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3-basic |
| 载体出品公司: | Promega |
| 空载体大小: | 4818 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 荧光素酶 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30°C |
| 宿主菌: | MACH1 |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | Arg1 promoter/enhancer -31/-3810 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 3779 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | 萤火虫荧光素酶（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | MluI（未知是否已销毁） |
| 3’克隆位点: | XhoI（未知是否已销毁） |
| 5’测序引物 | RVprimer3 |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGL3-mArg1 promoter/enhancer -31/-3810 was a gift from Peter Murray (Addgene plasmid # 34571 ; http://n2t.net/addgene:34571 ; RRID:Addgene_34571) |
| 参考文献部分: | Enhancer-mediated control of macrophage-specific arginase I expression. Pauleau AL, Rutschman R, Lang R, Pernis A, Watowich SS, Murray PJ. J Immunol. 2004 Jun 15;172(12):7565-73. PubMed 15187136 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL3-mArg1 promoter/enhancer -31/-3810 8566 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGL3-mArg1 promoter/enhancer -31/-3810
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8566)
AUTHORS Pauleau AL, Rutschman R, Lang R, Pernis A, Watowich SS, Murray PJ
TITLE Enhancer-mediated control of macrophage-specific arginase I
expression.
JOURNAL J Immunol. 2004 Jun 15;172(12):7565-73.
PUBMED 15187136
REFERENCE 2 (bases 1 to 8566)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8566
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(43..631)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(123..142)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(802..1662)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1425..1444
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1663..1767)
/gene="bla"
/label=AmpR promoter
rep_origin 1794..2249
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1881..1900)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2091..2112
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 2380..2428
/note="synthetic polyadenylation signal"
misc_feature 2442..2533
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 2482..2501
/label=RVprimer3
/note="pGL3 vector, forward primer"
regulatory 6370..6379
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6376..8028
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(6442..6460)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 8069..8190
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8080..8099)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 8134..8153
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(8438..8455)
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
1801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
1861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
1921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
1981 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
2041 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
2101 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
2161 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
2221 ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg
2281 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
2341 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
2401 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
2461 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
2521 agaacatttc tctatcgata ggtaccgagc tcttacgcgt agattgccag gaatatacca
2581 gacactggag ctaggtaaag gcctcctgta cacccataca aggagcgctt ctctgccttc
2641 aaatatcaac aagttttaaa tccccagtgt gtagtacaga gcaggctggg tggatttgag
2701 gtctgtgttc ttaggaattg aacctttaaa gcgcagtata cattctctac gctaaggaaa
2761 gacttgggct gggaaaagtg ggctctgcca agactcaggc cgatgacctc gtcccctcca
2821 gagcagtggg tctcaagctt cctaactctc caacctttta atacagttct tatgttgcag
2881 tggccccaat catcaaattg ttttgttgtt acagcataac tataattttc ctagttataa
2941 agcatactgt aaacaactgt gttttctgat ggttttaggg taaccgctgt gaaaggatct
3001 atcagcccca aaaggaattg ggatccacag gttaagaacc agtgccttag atgttctgag
3061 gccacagctt tggagattga aaagacagtc ctttgtgaag actagaatct taggccaagg
3121 tgcttgctgc caagcctgac accccagtct gactctcaga acccatacag caaggagaac
3181 ctcctcaggg ccatggtatg tgtttcccaa cacggacaca cacacaatca cacagagtaa
3241 agggtttttt aattaagcaa gaaatacagt ttacacatat gtctgcataa ggtcacggag
3301 ggtggtagcc gacgagagac cagctcatct tcaataagga agtcagagag cagaaggctt
3361 tgtcagcagg gcaagactat actttgttag gaagtgaggc attgttcaga cttccttatg
3421 ctttcttatg aacaggctgt attagccaac agtcctgtct gtacgtgagt tgtgccactt
3481 tgggttgagg gtgctgggct aacacagatc ttagttaagc atttccttgt cctttgattc
3541 ctcaatgacc cagaacaggc aaacaatacg atacttatgt ttgatgaatt agatgactgc
3601 atctacaact gaacttgtgc actgagttct ggatggtcgc ctgaaggaga acgcagggag
3661 gcaggcgata ctttaatagg actgactgtg agggtaaatc ggtgtcatac atggaaccgt
3721 cgtgtgtatg gagataccca gaacaataaa gacccaagaa aaacagatag tcaagaaatc
3781 atgagtttta cttaatcagg gcgttttgat cagtcagtcc aatgatgcca atgatgaaaa
3841 gtcaccctca aactgcccga gggaagactt tcaggaaaag ccctggtgat aaagtttaac
3901 agcaaatacc tatgaatgaa tgtataaagc tagtaacggc cgtagcactg ttttcaaaac
3961 gtagtcaccg gctcactcta catttgcgag accccgacag aagtaaatgt aaggtcaagc
4021 gattttgaaa aagaggagca ccagttcctt tcctgcagct ctaacgtcct accaatgggg
4081 ctgcatggac tggtgcagcc acaggtgatg agcccagagc atagaggaaa ctggaagatg
4141 gagcagcgta tcgtttataa cagcaacttg gcatctgatg gagatagctc agtggtaacg
4201 tggggagtct gtccatttgg tgctggtggg gaaactggga gagacaaagg ggaaatggag
4261 gatttaaaca aaatacaaaa aaaaaaaatg gacgcaacag tcagcaatca ctgtgaatag
4321 caaattcttc atgcatccaa gacttaagcc cagcatcttt caggcaggaa aacaatctac
4381 ccctgaatgg catttttctt cttttctttt tacctttggg gcaggttctg tgttgacctt
4441 tgaactaacc ctataacccc agcaggtgtt gaacctataa tccccctgcc tcatcctctc
4501 aaatagctgg aatttgtggg ccagcaccaa gaggcccaaa gaaagagaaa gtttaaatta
4561 aatagaagaa aatgtagagg tattgatagg tggtggtatc acacaccttt aatcccagca
4621 ctcaggaggc agtgacagat tgctatctgt gagttcaagg ttaggctggt ctatagggct
4681 agttctaggg tagccagagc cacacaaaga aaacatgtct ccaaaaagca aacaaaaatg
4741 tagaggtgca catttgggat ctgggagcag gtaaaactta aattcctagc tagcttcaaa
4801 tatataggaa aattctatct tacataaatc aattttttaa aaaaatctat aaccgtatat
4861 cagaaaaaca tagtaagatg aaaaatatat ttgaaaagga ggtaccaaaa tggtcctgag
4921 ggactttggc ttcttgattt attcattcaa taaatgcaat acatttattc agtcaataag
4981 tcaatgttta ttttgtattg aacgattcta tgtactgatg gtagagggaa cagaatcttt
5041 cttgaggtat taaaattctg ggtgcttttt gtcctcacaa ggttaaagaa atttacttct
5101 gacatgtggg ttttcttttt aaaattataa tcatgtaact ttgtaactgg gagaatactt
5161 ggttgtgttt cttctaaatt aatactttta aaaatatttc tttagtgttg ggggtggctt
5221 tacacaggga ccggaccaca tcacccacgt ggaggtcaga gatcaacttg gaggagttga
5281 ctctttcctt gcaccaaatg ggttctgcgg gtcaaactcg ggtcatcggg cttggtgtta
5341 ccggctggcc tctctcatct gccctagatc tcagggggcc ctgctgacac tctgctggtg
5401 tgtagattag aatcgcggca gctcggcggg tgcagaatgc ccattgctcc gttttgattc
5461 ttctgcaacc tgtatgtgac tagcaacctc accaagctgc agtctcttcc tcggcaaaac
5521 agcataagaa tgattctcag tctgaagtta gtttaaagag gaaacaggaa acgaaagaac
5581 attaactaga atagcacttg gcacacgaca gacaactcat attagctagt ttgctttccc
5641 tatcttggga tatcatgatt ccaaaaatga gattttcccg gagactgtat ttcaaaaata
5701 gaactgcttt gggttgtcag ggaaataaat gatgccttcc tgaaataata ataataataa
5761 caacaacaac aacaacaaca atataaaaca aaaaccagcc ccatgctttc ctagacagtg
5821 taacctggtg acacatgaaa tgtgtctcac tttccccaga acttgaagcc ttgactcagg
5881 atgctcaaca ggagggaagt aagagacacc accccccacc cccgcttgct gttttagcct
5941 caccttgcaa gtccccagtg gacttaaatc ttggaaaagg tgagcaccct gccctgaggt
6001 gcccaggccg gaagcctagc acttcacatg aggttatgaa atcacacata attgtcaatt
6061 gtctgaggag agattaatgt catccagctg gctttttcaa aagggtgtga actggacgga
6121 tgaataatgc tcagagggaa gaatggtagt tcctctgatg gggaggttct gttgactctg
6181 tcattcttcc attcggtggg cggagccagt tgttggataa acagatccaa cctgattata
6241 aggggggaaa aagatgtgcc ctctgtcttt tagggttacg gccggtggag aggagctgga
6301 cagcccgagc acatgcagcc tcgagatctg cgatctaagt aagcttggca ttccggtact
6361 gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
6421 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
6481 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
6541 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
6601 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
6661 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
6721 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
6781 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
6841 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
6901 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
6961 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
7021 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
7081 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
7141 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
7201 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
7261 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
7321 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
7381 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
7441 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
7501 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
7561 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
7621 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
7681 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
7741 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
7801 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
7861 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
7921 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
7981 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg
8041 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
8101 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
8161 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
8221 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
8281 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
8341 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
8401 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
8461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
8521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggcca
//
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